To add to what Ju-nie told me yesterday,
we'll be conducting P2 (or is it microplate reading?) on both scrambled and mismatch DNA aptamer sequence to confirm it's specificity.
Scrambled: Random arrangement of DNA sequences by computer software
Mismatch: Difference only in certain nucleotide bases.
There maybe a consensus sequence between the mismatch and the true DNA which maybe identified through the increment of fluorescent intensity or computer system.

haven't understood the ppt yet. more time needed.